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C7H14O Proteinase K In Stock With Reasonable Price


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  • Product Description
    Proteinase K Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It is a broad spectrum endopeptidase with a very high specific activity. Proteinase K Molecular Biology Grade is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS. As a result, this enzyme is widely using for digestion of proteins, including DNases and RNases, during nucleic acid preparations without compromising the integrity of isolated DNA or RNA. Proteinase K Molecular Biology Grade is free of exonucleases, endonucleases, and ribonucleases. Also check our Proteinase K NGS Grade
    Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
    High activity and exceptional purity (Molecular Biology Grade & NGS Grade).
    Active at high temperatures (up to 56°C) and denaturing conditions (e.g. in the presence of urea and/or SDS), what makes it ideal
    for digesting proteins in variety of applications.
    Stable over a wide pH range: 4.0–12.5 (optimum pH 7.5–8.0).
    Decreased amount of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
    Available as powder, lyophilized “cake” or liquid.
    Extraction of DNA and RNA from different starting materials.
    Purification of target material from contaminating proteins.
    Removal of DNases and RNases during nucleic acids isolation.
    One unit of Proteinase K hydrolyzes urea-denaturated hemoglobin producing color equivalent of 1 μmol tyrosine per 1 min at 37°C
    and pH 7.5 (Folin & Ciocalteu’s method), 1 U = 1 mAnsonU.
    Proteinase K – Lyophilized powder, NGS Grade
    DNA content : ≤ 0.1 pg/mg by qPCR

    Solubility in water : ≥ 50 mg/ml

    ≥ 35 U/mg lyophilizate
    ≥ 45 U/mg protein

    Protein content : ≥ 70% Protein content is determined by measuring absorbance at 280 nm.

    Stock solution preparation 20-50 mg/ml solutions: use purified water for immediate use, or 50% glycerol in purified water (v/v)
    for long-term storage at -20˚C. < 20 mg/ml solutions: use 50 mM Tris-HCl, pH = 7.8, 3 mM CaCl2 for immediate use, or 50 mM
    Tris-HCl, pH = 7.8, 3 mM CaCl2 , 50% glycerol for long-term storage at -20˚C.
    Proteinase K is a powerful proteolytic enzyme isolated from Candida albicans. It has a high specific activity and is a key reagent for DNA extraction.

    The enzyme is active in a wide pH range (4~12.5) and high temperature (50~70°C), and is used for the isolation of plasmid or genomic DNA and RNA.

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